Journal: Viruses

Article Title: A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus

doi: 10.3390/v8080227

Figure Lengend Snippet: Genotype 1 VP13 protein reacts with the monoclonal antibody (Mab) in western blotting (WB). ( A ) Detection of purified maltose-binding protein (MBP)-VP13, but not MBP-X or MBP-Helicase. MBP detection was done as a control; ( B ) Detection of only genotype 1 VP13 in lysate of HEK293 cells transiently transfected with YFP-VP13 plasmids or empty vector (EV). Purified MBP-VP13 was included as a positive control. Ab: antibody.

Article Snippet: Antibodies used in the WB were rabbit anti-MBP antibody (Rockland Immunochemicals Inc.), mouse anti-YFP (yellow fluorescent protein) monoclonal antibody (Rockland Immunochemicals Inc.), mouse anti-tubulin monoclonal antibody (Sigma-Aldrich Corp., St. Louis, MO, USA), homemade monoclonal anti-VP13 clone 3C3 (this study) and goat anti-mouse IgG or goat anti-rabbit IgG conjugated with horseradish peroxidase (Rockland Immunochemicals Inc.).

Techniques: Western Blot, Purification, Binding Assay, Control, Transfection, Plasmid Preparation, Positive Control